Interaction of a Novel Platinum Drug With Bovine Serum Albumin: Ftir and Uv-Vis Spectroscopy Analysis

dc.authorid Korkmaz, Filiz/0000-0003-3512-3521
dc.authorid Altunoz Erdogan, Deniz/0000-0003-1071-4173
dc.authorid Ozalp Yaman, Seniz/0000-0002-4166-0529
dc.authorscopusid 8664101000
dc.authorscopusid 56059422300
dc.authorscopusid 56054555600
dc.authorwosid Korkmaz, Filiz/GOH-1457-2022
dc.authorwosid Erdogan, Deniz Altunoz/B-3268-2016
dc.authorwosid Yaman, Şeniz Özalp/AAK-1854-2021
dc.contributor.author Korkmaz, Filiz
dc.contributor.author Erdogan, Deniz Altunoz
dc.contributor.author Ozalp-Yaman, Seniz
dc.contributor.other Physics Group
dc.contributor.other Chemical Engineering
dc.date.accessioned 2024-07-05T14:32:11Z
dc.date.available 2024-07-05T14:32:11Z
dc.date.issued 2015
dc.department Atılım University en_US
dc.department-temp [Korkmaz, Filiz] Atilim Univ, Biophys Lab, Fac Engn, TR-06836 Ankara, Turkey; [Erdogan, Deniz Altunoz; Ozalp-Yaman, Seniz] Atilim Univ, Dept Chem Engn & Appl Chem, Fac Engn, TR-06836 Ankara, Turkey en_US
dc.description Korkmaz, Filiz/0000-0003-3512-3521; Altunoz Erdogan, Deniz/0000-0003-1071-4173; Ozalp Yaman, Seniz/0000-0002-4166-0529 en_US
dc.description.abstract Platinum complexes have proven to be very effective in cancer treatment. However, severe side effects of these drugs have lead scientists to pursue new platinum complex derivatives. A novel blue platinum compound, called Platinum-Blue (Pt-Blue), is one of the promising candidate platinum compounds to be used for tumor treatment. In this study, the interaction of Pt-Blue with bovine serum albumin (BSA) has been investigated using UV-Vis and FTIR spectroscopy. One of the findings is that the drug-protein interaction type depends on the drug concentration. Though Pt-Blue is attached to the surface of BSA at high concentrations, it interacts with a hydrophobic region of the protein at low concentrations with a binding constant of 1.93 x 10(5) M-1. Spectroscopic results indicate the hydrophobic docking position to be around Trp 213 in domain II, which is surrounded by a number of Asp and Glu. During this interaction, helices such as helix-10, helix-18, helix-19 and helix-24 change orientation and/or partially unfold to make room for the compound. Binding constants at high and low concentrations of Pt-Blue are determined using UV-Vis spectroscopy, which are found to be comparable to cisplatin. FTIR spectroscopy also reveals that the interaction between Pt-Blue and BSA is noncovalent, which makes the candidate drug favorable because it is available for DNA binding while being carried by albumin. en_US
dc.description.sponsorship Atilim University [ATU-ALP-1011-01, ATU-BAP/2006/04] en_US
dc.description.sponsorship This work is supported by the grants ATU-ALP-1011-01 and ATU-BAP/2006/04 provided by Atilim University. We thank Prof. Dr Erik Goormaghtigh from Universite Libre de Bruxelles, Belgium, for generously providing us with the software Kinetics for side chain subtraction. The authors also acknowledge Dr Hakan Kayi for his valuable contributions during the preparation of the manuscript. en_US
dc.identifier.citationcount 25
dc.identifier.doi 10.1039/c5nj00785b
dc.identifier.endpage 5685 en_US
dc.identifier.issn 1144-0546
dc.identifier.issn 1369-9261
dc.identifier.issue 7 en_US
dc.identifier.scopus 2-s2.0-84934323924
dc.identifier.scopusquality Q3
dc.identifier.startpage 5676 en_US
dc.identifier.uri https://doi.org/10.1039/c5nj00785b
dc.identifier.uri https://hdl.handle.net/20.500.14411/756
dc.identifier.volume 39 en_US
dc.identifier.wos WOS:000357106400083
dc.identifier.wosquality Q2
dc.institutionauthor Korkmaz Özkan, Filiz
dc.institutionauthor Özalp Yaman, Şeniz
dc.language.iso en en_US
dc.publisher Royal Soc Chemistry en_US
dc.relation.publicationcategory Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı en_US
dc.rights info:eu-repo/semantics/closedAccess en_US
dc.scopus.citedbyCount 27
dc.subject [No Keyword Available] en_US
dc.title Interaction of a Novel Platinum Drug With Bovine Serum Albumin: Ftir and Uv-Vis Spectroscopy Analysis en_US
dc.type Article en_US
dc.wos.citedbyCount 28
dspace.entity.type Publication
relation.isAuthorOfPublication 1ebbd479-dfc4-411f-8702-e06ba4616da2
relation.isAuthorOfPublication f3a864db-9deb-4073-bcf2-7569cbfd9610
relation.isAuthorOfPublication.latestForDiscovery 1ebbd479-dfc4-411f-8702-e06ba4616da2
relation.isOrgUnitOfPublication 2682824b-512d-4a4e-8498-5b5719f606fe
relation.isOrgUnitOfPublication bebae599-17cc-4f0b-997b-a4164a19b94b
relation.isOrgUnitOfPublication.latestForDiscovery 2682824b-512d-4a4e-8498-5b5719f606fe

Files

Collections