Evaluation of the Efficacy of Silymarin and Dexmedetomidine on Kidney and Lung Tissue in the Treatment of Sepsis in Rats With Cecal Perforation

dc.authorid Yavuz, Aydın/0000-0003-0091-7997
dc.authorid Yığman, Zeynep/0000-0003-1985-9280
dc.authorid Dursun, Ali Dogan/0000-0001-9056-0025
dc.authorwosid Yavuz, Aydın/AAI-5091-2020
dc.authorwosid Yığman, Zeynep/HTM-5876-2023
dc.authorwosid Dursun, Ali Dogan/AAH-7617-2019
dc.contributor.author Yavuz, Aydin
dc.contributor.author Kucuk, Aysegul
dc.contributor.author Ergorun, Aydan Iremnur
dc.contributor.author Dursun, Ali Dogan
dc.contributor.author Yigman, Zeynep
dc.contributor.author Alkan, Metin
dc.contributor.author Arslan, Mustafa
dc.contributor.other Basic Sciences
dc.date.accessioned 2024-07-05T15:23:16Z
dc.date.available 2024-07-05T15:23:16Z
dc.date.issued 2024
dc.department Atılım University en_US
dc.department-temp [Yavuz, Aydin] Gazi Univ, Fac Med, Dept Gen Surg, TR-06510 Ankara, Turkiye; [Kucuk, Aysegul] Kutahya Hlth Sci Univ, Fac Med, Dept Physiol, TR-43020 Kutahya, Turkiye; [Ergorun, Aydan Iremnur; Alkan, Metin; Arslan, Mustafa] Gazi Univ, Fac Med, Dept Anesthesiol & Reanimat, 29 Mevlana Blvd, TR-06510 Ankara, Turkiye; [Dursun, Ali Dogan] Atilim Univ, Fac Med, Dept Physiol, TR-06830 Ankara, Turkiye; [Yigman, Zeynep] Gazi Univ, Fac Med, Dept Histol & Embryol, TR-06510 Ankara, Turkiye; [Yigman, Zeynep] Gazi Univ, Neurosci & Neurotechnol Ctr Excellence, TR-06510 Ankara, Turkiye; [Arslan, Mustafa] Gazi Univ, Life Sci Applicat & Res Ctr, TR-06830 Ankara, Turkiye; [Arslan, Mustafa] Gazi Univ, Lab Anim Breeding & Expt Res Ctr, TR-06510 Ankara, Turkiye en_US
dc.description Yavuz, Aydın/0000-0003-0091-7997; Yığman, Zeynep/0000-0003-1985-9280; Dursun, Ali Dogan/0000-0001-9056-0025 en_US
dc.description.abstract Sepsis is a systemic inflammatory response syndrome that develops in the host against microorganisms. This response develops away from the primary infection site and results in end-organ damage. The present study aimed to investigate the protective and therapeutic effects on lung and kidney tissue of silymarin (S) and dexmedetomidine (DEX) applied 1 h before and after sepsis induced by the cecal ligation and puncture (CLP) method in rats. A total of 62 rats was randomly divided into eight groups: i) Control (n=6); ii) cecal perforation (CLP; n=8); iii) S + CLP (n=8; S + CLP; S administered 1 h before CPL); iv) CLP + S (n=8; S administered 1 h after CLP); v) DEX + CLP (n=8; D + CLP; DEX administered 1 h before CLP); vi) CLP + D (n=8; DEX administered 1 h after CLP); vii) SD + CLP (n=8; S and DEX administered 1 h before CLP) and viii) CLP + SD (n=8; S and DEX administered 1 h after CLP). After the cecum filled with stool, it was tied with 3/0 silk under the ileocecal valve and the anterior surface of the cecum was punctured twice with an 18-gauge needle. A total of 100 mg/kg silymarin and 100 mu g/kg DEX were administered intraperitoneally to the treatment groups. Lung and kidney tissue samples were collected to evaluate biochemical and histopathological parameters. In the histopathological examination, all parameters indicating kidney injury; interstitial edema, peritubular capillary dilatation, vacuolization, ablation of tubular epithelium from the basement membrane, loss of brush border in the proximal tubule epithelium, cell swelling and nuclear defragmentation; were increased in the CLP compared with the control group. Silymarin administration increased kidney damage, including ablation of tubular epithelium from the basement membrane, compared with that in the CLP group. DEX significantly reduced kidney damage compared with the CLP and silymarin groups. The co-administration of DEX + silymarin decreased kidney damage, although it was not as effective as DEX-alone. To conclude, intraperitoneal DEX ameliorated injury in CLP rats. DEX + silymarin partially ameliorated injury but silymarin administration increased damage. As a result, silymarin has a negative effects with this dosage and DEX has a protective effect. In the present study, it was determined that using the two drugs together had a greater therapeutic effect than silymarin and no differences in the effects were not observed any when the application times of the agents were changed. en_US
dc.identifier.citationcount 0
dc.identifier.doi 10.3892/etm.2024.12530
dc.identifier.issn 1792-0981
dc.identifier.issn 1792-1015
dc.identifier.issue 6 en_US
dc.identifier.pmid 38655036
dc.identifier.uri https://doi.org/10.3892/etm.2024.12530
dc.identifier.uri https://hdl.handle.net/20.500.14411/2293
dc.identifier.volume 27 en_US
dc.identifier.wos WOS:001207226400001
dc.identifier.wosquality Q3
dc.institutionauthor Dursun, Ali Doğan
dc.institutionauthor Yığman, Zeynep
dc.language.iso en en_US
dc.publisher Spandidos Publ Ltd en_US
dc.relation.publicationcategory Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı en_US
dc.rights info:eu-repo/semantics/openAccess en_US
dc.subject cecal ligation and puncture en_US
dc.subject sepsis en_US
dc.subject lung en_US
dc.subject kidney en_US
dc.subject dexmedetomidine en_US
dc.subject silymarin en_US
dc.title Evaluation of the Efficacy of Silymarin and Dexmedetomidine on Kidney and Lung Tissue in the Treatment of Sepsis in Rats With Cecal Perforation en_US
dc.type Article en_US
dc.wos.citedbyCount 1
dspace.entity.type Publication
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