Özge Boyacıoğlu

Boyacıoğlu, Özge

Name Variants

Boyacioglu,Ozge
Boyacioglu,Ö.
Boyacioglu, Ozge
Özge Boyacıoğlu
B., Özge
Boyacioglu,O.
Ö.,Boyacıoğlu
B.,Özge
Ozge, Boyacioglu
Boyacıoğlu, Özge
Ö., Boyacıoğlu
B.,Ozge
B., Ozge
Boyacioglu O.
O.,Boyacioglu
Boyacıoğlu,Ö.
O., Boyacioglu
Özge, Boyacıoğlu
Boyacioglu, OEzge

Job Title

Araştırma Görevlisi

Email Address

ozge.boyacioglu@atilim.edu.tr

Scholarly Output

8

Articles

5

Citation Count

52

Supervised Theses

0 Scholarly Output Search Results

Now showing 1 - 8 of 8

Citation Count: 2
Thioredoxin System and miR-21, miR-23a/b and let-7a as Potential Biomarkers for Brain Tumor Progression: Preliminary Case Data
(Elsevier Science inc, 2022) Kılıç, Nedret; Boyacıoğlu, Özge; Saltoglu, Gamze Turna; Bulduk, Erkut Baha; Kurt, Gokhan; Korkusuz, Petek; Basic Sciences; Surgical Sciences
BACKGROUND: The thioredoxin system and microRNAs (miRNAs) are potential targets for both cancer progression and treatment. However, the role of miRNAs and their relation with the expression profile of thioredoxin system in brain tumor progression remains unclear. METHODS: In this study, we aimed to determine the expression profiles of redox components Trx-1, TrxR-1 and PRDX-1, and oncogenic miR-21, miR-23a/b and let-7a and oncosuppressor miR-125 in different brain tumor tissues and their association with increasing tumor grade. We studied Trx-1, TrxR-1, and PRDX-1 messenger RNA expression levels by quantitative real-time polymerase chain reaction and protein levels by Western blot and miR-23a, miR-23b, miR-125a, miR-21, and let-7a miRNA expression levels by quantitative real-time polymerase chain reaction in 16 glioma, 15 meningioma, 5 metastatic, and 2 benign tumor samples. We also examined Trx-1, TrxR-1, and PRDX-1 protein levels in serum samples of 36 patients with brain tumor and 37 healthy volunteers by enzyme-linked immunosorbent assay. RESULTS: We found that Trx-1, TrxR-1, and PRDX-1 presented high messenger RNA expression but low protein expression in low-grade brain tumor tissues, whereas they showed higher protein expression in sera of patients with low-grade brain tumors. miR-23b, miR-21, miR-23a, and let-7a were highly expressed in low-grade brain tumor tissues and positively correlated with the increase in thioredoxin system activity. CONCLUSIONS: Our findings showed that Trx-1, TrxR-1, miR-21, miR-23a/b, and let-7a might be used for brain tumor diagnosis in the clinic. Further prospective studies including molecular pathway analyses are required to validate the miRNA/Trx system regulatory axis in brain tumor progression.
Citation Count: 23
Architecture of bone tissue and its adaptation to pathological conditions
(Elsevier, 2020) Boyacıoğlu, Özge; Boyacıoğlu,Ö.; Gizer,M.; Korkusuz,P.; Korkusuz,F.; Basic Sciences
Bone tissue is a mineralized and viscous-elastic connective tissue, which exerts crucial functions in our body such as support and protection of other tissues and mineral storage. Bone can adapt itself through a remodeling process, which is controlled by its cells, various local and systemic factors. It is a very complicated process composed of both cellular reactions and its effects on the internal structure of the bone. An imbalance between bone resorption and formation due to disease may alter its structure and mechanics. Mechanical properties of bone tissue are affected by different loading grades. Collagen material found in the extracellular matrix gives bone its elasticity. Bone is, nevertheless, a fragile structure depending on the loading and mineral content that also strengthens the bone. In this chapter, the structure of the bone in micro and macro scale, its mechanical properties in physiological circumstances and adaptation to pathological conditions will be discussed. © 2020 Elsevier Inc. All rights reserved.
Citation Count: 0
Clinic-oriented injectable smart material for the treatment of diabetic wounds: Coordinating the release of GM-CSF and VEGF
(Elsevier, 2024) Boyacıoğlu, Özge; Kalaycioglu, Gokce Dicle; Boyacioglu, Ozge; Korkusuz, Petek; Aydogan, Nihal; Vargel, Ibrahim; Basic Sciences
Chronic wounds are often caused by diabetes and present a challenging clinical problem due to vascular problems leading to ischemia. This inhibits proper wound healing by delaying inflammatory responses and angiogenesis. To address this problem, we have developed injectable particle-loaded hydrogels which sequentially release Granulocyte-macrophage- colony-stimulating-factor (GM-CSF) and Vascular endothelial growth factor (VEGF) encapsulated in polycaprolactone-lecithin-geleol mono-diglyceride hybrid particles. GM-CSF promotes inflammation, while VEGF facilitates angiogenesis. The hybrid particles (200 -1000 nm) designed within the scope of the study can encapsulate the model proteins Bovine Serum Albumin 65 +/- 5 % and Lysozyme 77 +/- 10 % and can release stably for 21 days. In vivo tests and histological findings revealed that in the hydrogels containing GM-CSF/VEGF-loaded hybrid particles, wound depth decreased, inflammation phase increased, and fibrotic scar tissue decreased, while mature granulation tissue was formed on day 10. These findings confirm that the hybrid particles first initiate the inflammation phase by delivering GM-CSF, followed by VEGF, increasing the number of vascularization and thus increasing the healing rate of wounds. We emphasize the importance of multi-component and sequential release in wound healing and propose a unifying therapeutic strategy to sequentially deliver ligands targeting wound healing stages, which is very important in the treatment of the diabetic wounds.
Citation Count: 0
Development and validation of a sensitive assay for the quantification of arachidonoylcyclopropylamide (ACPA) in cell culture by LC-MS/MS
(Springer int Publ Ag, 2023) Boyacıoğlu, Özge; Recber, Tuba; Kir, Sedef; Korkusuz, Petek; Nemutlu, Emirhan; Basic Sciences
Synthetic and natural cannabinoid derivatives are highly investigated as drug candidates due to their antinociceptive, antiepileptic and anticancer potential. Arachidonoylcyclopropylamide (ACPA) is a synthetic cannabinoid with antiproliferative and apoptotic effects on non-small cell lung cancer and pancreatic and endometrial carcinoma. Thus, ACPA has a great potential for being used as an anticancer drug for epithelial cancers. Therefore, determining the levels of ACPA in biological fluids, cells, tissues and pharmaceutical dosage forms is crucial in monitoring the effects of various pharmacological, physiological and pathological stimuli on biological systems. However, the challenge in the quantification of ACPA is its short half-life and lack of UV signal. Therefore, we developed a liquid chromatography-tandem mass spectrometric (LC-MS/MS) method for sensitive and selective quantification of ACPA in cell culture medium and intracellular matrix. Multiple reaction monitoring in the positive ionization mode was used for detection with 344 -> 203 m/z transitions. The separation of ACPA was performed on C18 column (50 x 3.0 mm, 2.1 mu m) with the mobile phase run in the gradient mode with 0.1% formic acid (FA) in water and 0.1% FA in acetonitrile at a flow rate of 0.3 ml/min. The assay was linear in the concentration range of 1.8-1000 ng/mL (r = 0.999). The validation studies revealed that the method was linear, sensitive, accurate, precise, selective, repeatable, robust and rugged. Finally, the developed method was applied to quantify ACPA in cell culture medium and intracellular matrix.
Citation Count: 7
From nutrition to medicine: Assessing hemorrhoid healing activity of Solanum melongena L. via in vivo experimental models and its major chemicals
(Elsevier Ireland Ltd, 2020) Boyacıoğlu, Özge; Yalçın,F.N.; Boyacıoğlu,Ö.; Korkusuz,P.; Akkol,E.K.; Nemutlu,E.; Çalışkan,U.K.; Basic Sciences
Ethnopharmacological relevance: Solanum melongena L. (eggplant) is used for treatment of rheumatism, beriberi, itching, toothache, bleeding, asthma, bronchitis, cholera, neuralgia and hemorrhoids in traditional medicine (Turkish, Chinese, and Indian). Hemorrhoids from these diseases, are common illness in all over the world, which are treated with various approaches including ethnobotanicals. Aim of the study: This study aimed to evaluate the anti-hemorrhoidal activity of eggplant, an edible plant, which is commonly utilized around the world. Materials & methods: In vivo anti-hemorrhoidal activity of the methanolic extract prepared from eggplant was evaluated by experimental hemorrhoid model, subsequently histological and biochemical analysis. Hemorrhoid, which was induced by applying croton oil to the anal area of the rats. Furthermore, the extract was screened for anti-inflammatory activity which is based on the inhibition of acetic acid-induced increase in capillary permeability. The healing potential was comparatively assessed with a reference Pilex® tablet and cream. Phytochemical analysis performed by HPLC. The amount of the major phenolic compound (chlorogenic acid) in extract was found by using HPLC method. Results: Histological and biochemical analysis demonstrated that eggplant extract is highly effective against hemorrhoid in comparison to the controls and the commercial preparation. In addition, the methanolic extract demonstrated significant inhibitory effect on acetic acid-induced increase in capillary permeability. The phytochemical studies identified major compound as chlorogenic acid (2.86%) by liquid chromatography. Conclusion: The eggplant calyxes, not edible, are easy to reach, by products/vast from the food sources. This is the first scientific evidence revealing that the eggplant extract has significant anti-hemorrhoidal and anti-inflammatory activity. © 2020 Elsevier B.V.
Citation Count: 2
Cannabinoids as Prospective Anti-Cancer Drugs: Mechanism of Action in Healthy and Cancer Cells
(Springer, 2023) Boyacıoğlu, Özge; Korkusuz,P.; Basic Sciences
Endogenous and exogenous cannabinoids modulate many physiological and pathological processes by binding classical cannabinoid receptors 1 (CB1) or 2 (CB2) or non-cannabinoid receptors. Cannabinoids are known to exert antiproliferative, apoptotic, anti-migratory and anti-invasive effect on cancer cells by inducing or inhibiting various signaling cascades. In this chapter, we specifically emphasize the latest research works about the alterations in endocannabinoid system (ECS) components in malignancies and cancer cell proliferation, migration, invasion, angiogenesis, autophagy, and death by cannabinoid administration, emphasizing their mechanism of action, and give a future perspective for clinical use. © 2022, The Author(s), under exclusive license to Springer Nature Switzerland AG.
Citation Count: 2
Trace Element Containing Nano-HAp for Preventing Musculoskeletal Infections
(Springer Science and Business Media B.V., 2021) Boyacıoğlu, Özge; Boyacıoğlu,Ö.; Korkusuz,P.; Korkusuz,F.; Basic Sciences
Musculoskeletal infections are difficult to diagnose and rapidly reach the chronic phase that is difficult to treat. Osteomyelitis and septic arthritis are inflammatory musculoskeletal diseases and their recovery should contain bone or joint regeneration approaches. Nanometer-sized hydroxyapatite is the main inorganic component of the bone tissue that resembles its extracellular matrix. Nanometer-sized hydroxyapatite composite is also an efficient carrier for various regenerative molecules and medicine. Trace elements on the other hand enhance bone formation, mineralization and have antibacterial properties. Bioactivity, biocompatibility, and antibacterial properties of nanometer-sized hydroxyapatite can be gained and improved with trace elements such as zinc, boron, magnesium, strontium, and molybdenum. This chapter summarizes studies on the effects of zinc, boron, magnesium, strontium, and/or molybdenum-doped nanometer-sized hydroxyapatite that can be used to treat musculoskeletal infections. © 2021, The Author(s), under exclusive license to Springer Nature Switzerland AG.
Citation Count: 16
ACPA decreases non-small cell lung cancer line growth through Akt/PI3K and JNK pathways in vitro
(Springernature, 2021) Boyacıoğlu, Özge; Bilgic, Elif; Varan, Cem; Bilensoy, Erem; Nemutlu, Emirhan; Sevim, Duygu; Korkusuz, Petek; Basic Sciences
Therapeutic agents used for non-small cell lung cancer (NSCLC) have limited curative efficacy and may trigger serious adverse effects. Cannabinoid ligands exert antiproliferative effect and induce apoptosis on numerous epithelial cancers. We confirmed that CB1 receptor (CB1R) is expressed in NSCLC cells in this study. Arachidonoylcyclopropylamide (ACPA) as a synthetic, CB1R-specific ligand decreased proliferation rate in NSCLC cells by WST-1 analysis and real-time proliferation assay (RTCA). The half-maximal inhibitory concentration (IC50) dose of ACPA was calculated as 1.39x10(-12)M. CB1 antagonist AM281 inhibited the antiproliferative effect of ACPA. Flow cytometry and ultrastructural analyzes revealed significant early and late apoptosis with diminished cell viability. Nano-immunoassay and metabolomics data on activation status of CB1R-mediated pro-apoptotic pathways found that ACPA inhibited Akt/PI3K pathway, glycolysis, TCA cycle, amino acid biosynthesis, and urea cycle and activated JNK pathway. ACPA lost its chemical stability after 24hours tested by liquid chromatography-mass spectrometry (LC-MS/MS) assay. A novel ACPA-PCL nanoparticle system was developed by nanoprecipitation method and characterized. Sustained release of ACPA-PCL nanoparticles also reduced proliferation of NSCLC cells. Our results demonstrated that low dose ACPA and ACPA-PCL nanoparticle system harbor opportunities to be developed as a novel therapy in NSCLC patients that require further in vivo studies beforehand to validate its anticancer effect.

Boyacıoğlu, Özge

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Scholarly Output

8

Articles

5

Citation Count

52

Supervised Theses

0

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