Browsing by Author "Ozer, A."

Now showing 1 - 2 of 2

In-Vivo Antioxidant and Therapeutic Effects of Ellagic Acid on Ischemia-Reperfusion Injury in Skeletal Muscle
(Turkish National Vascular and Endovascular Surgery Society, 2025) Demirtas, H.; Ozer, A.; Yigit, D.; Dursun, A.D.; Yigman, Z.; Kosa, C.; Arslan, M.
Aim: Skeletal muscle ischemia-reperfusion (IR) injury is a critical clinical issue characterized by oxidative stress, inflammation, and tissue damage, potentially leading to systemic organ dysfunction. Ellagic acid (EA), a naturally occurring polyphenolic compound, is widely recognized for its strong antioxidative, anti-inflammatory, and antiapoptotic effects demonstrated in various preclinical studies. This study sought to assess the therapeutic effects of EA in a rat model of lower extremity IR injury, focusing on histopathological and biochemical parameters. Material and Methods: 24 male Albino Wistar rats were randomly divided into four groups: Sham, EA, IR, and IR+EA. IR injury was induced by occluding the infrarenal abdominal aorta for 45 minutes, followed by 120 minutes of reperfusion. EA (40 mg/kg) was administered intraperitoneally prior to reperfusion. Left gastrocnemius muscle samples were collected for histopathological and biochemical analyses, including TOS, TAS, OSI, levels and PON-1 enzyme activity. Results: The IR group showed marked muscle injury, with a significantly higher total injury score (10.00±0.63) compared to the Sham (2.00±0.58) and EA groups (2.00±0.52) (p<0.001, both). The IR-EA group demonstrated notable improvement, with a reduced total injury score (6.17±0.54), which was also significantly lower than the IR group (p<0.001). Biochemically, TAS levels and PON-1 activity significantly decreased while TOS and OSI levels increased in the IR group compared to the sham and EA groups. In addition, EA treatment significantly increased TAS levels and PON-1 activity while reducing TOS and OSI levels in the IR-EA group compared to the IR group (p=0.039, p=0.045, p=0.045, p=0.007, respectively). Conclusion: EA effectively mitigated skeletal muscle damage induced by IR injury through its antioxidative, anti-inflammatory, and antiapoptotic mechanisms. The results suggest that EA exhibits potential effects as a therapeutic agent in managing IR-related injuries. © 2025, Turkish National Vascular and Endovascular Surgery Society. All rights reserved.
Citation - WoS: 1
Ozone Administration Reduces Myocardial Ischemia Reperfusion Injury in Streptozotocin-Induced Diabetes Mellitus Rat Model
(Baycinar Medical Publishing, 2024) Gulcan, M.B.; Demirtas, H.; Ozer, A.; Yıgman, Z.; Dursun, A.D.; Arslan, M.; Oktar, G.L.; Basic Sciences
Objective: This study aimed to investigate the effects of ozone therapy on myocardial ischemia/reperfusion injury in a diabetic rat model. Methods: The experimental study included 38 male Wistar Albino rats weighing between 200 and 250 g. The rats were randomly assigned to five groups. The sham group included six rats, while the other groups had eight rats each. The other groups were the diabetic ozone group, the diabetic group, the diabetic ischemia/reperfusion group (DIR), and the diabetic ischemia/reperfusion ozone group (DIRO). A total of 32 rats received 65 mg/kg streptozotocin, and a week after the administration, diabetes was confirmed by measuring blood sugar. The rats were fed ad libitum for 40 days to reveal macrovascular complications of diabetes. Malondialdehyde, catalase, superoxide dismutase, paraoxonase-1, total oxidative status, total antioxidant status, and oxidative stress index were assessed. A TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) assay was employed to assess apoptosis. Results: Histologic and biochemical assessments showed the benefits of ozone in myocardial ischemia/reperfusion injury in diabetic rats. The DIRO group was found to be superior to the DIR group. Conclusion: Ozone has cardioprotective effects in streptozotocin-induced diabetic rats through its antioxidant properties against oxidative stress. The study is unique in terms of ozone’s protective effects in diabetic rats against myocardial ischemia/reperfusion injury. However, further studies are needed to confirm our findings. © (2024), (Baycinar Medical Publishing). All rights reserved.